human oral epithelial cells hoec  (Celprogen Inc)

Journal: Cell Death & Disease

Article Title: SALL4 promotes cancer stem-like cell phenotype and radioresistance in oral squamous cell carcinomas via methyltransferase-like 3-mediated m6A modification

doi: 10.1038/s41419-024-06533-9

Figure Lengend Snippet: A Results based on the TCGA databases showed the expression level of methyltransferase (METTL3, METTL14, WTAP), demethyltransferase (ALKBH5, FTO). B Western blotting analysis showed the protein levels of METTL3, METTL14, WTAP, ALKBH5, FTO in OSCC cell lines (SCC4, SCC9, SCC15, CAL27) and HOEC. C qRT-PCR analysis of METTL3 in OSCC cells. D Disease-free survival rate analysis based on the GEPIA platform ( http://gepia.cancer-pku.cn/ ). E METTL3 expression levels based on tumor grade. F METTL3 expression levels based on nodal metastasis status. G , H Immunofluorescence images and western blot showing the METTL3 protein expression level in radioresistant and parental cells.

Article Snippet: Both SCC4 cell lines and human oral epithelial cell (HOEC) were purchased from the China Center for Type Culture Collection.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, Immunofluorescence

Journal: Turkish Journal of Medical Sciences

Article Title: Exosome-mediated long noncoding RNA (lncRNA) PART1 suppresses malignant progression of oral squamous cell carcinoma via miR-17-5p/SOCS6 axis

doi: 10.55730/1300-0144.5625

Figure Lengend Snippet: LncRNA PART1 was suppressed in OSCC A: Public database GEPIA ( http://gepia.cancer-pku.cn/ ). Displayed lncRNA PART1 expression in HNSCC tissue samples. B: Kaplan-Meier analysis on GEPIA C: RT-qPCR examined lncRNA PART1 expressions in OSCC cells and HOECs. *p =< 0.032, **p =< 0.0021, ***p =< 0.0002, and ****p < 0.0001.

Article Snippet: Human tongue squamous cell carcinoma cell lines (SCC9 and CAL27) and human normal oral epithelial cells (HOECs) were obtained from American type culture collection (ATCC, USA) and Procell (Wuhan, China), respectively.

Techniques: Expressing, Quantitative RT-PCR

Journal: Turkish Journal of Medical Sciences

Article Title: Exosome-mediated long noncoding RNA (lncRNA) PART1 suppresses malignant progression of oral squamous cell carcinoma via miR-17-5p/SOCS6 axis

doi: 10.55730/1300-0144.5625

Figure Lengend Snippet: LncRNA PART1 regulated miR-17-5p/SOCS6 to inactivate STAT3 signaling pathway A: SOCS6 mRNA expression in OSCC cells and HOECs were analyzed using RT-qPCR. B: TargetScan provided binding sites between miR-17-5p and SOCS6. C: Luciferase reporter test. D–F: Western blot assays were performed to measure the changes in SOCS6 protein expression. G. SOCS6 mRNA expression in SCC9 and CAL27 cells with PART1 overexpression and additional upregulation of miR-17-5p. H–J: Phosphorylated STAT3 and STAT3 proteins were validated by western blot. *p =< 0.032, **p =< 0.0021, ***p =< 0.0002, and ****p < 0.0001.

Article Snippet: Human tongue squamous cell carcinoma cell lines (SCC9 and CAL27) and human normal oral epithelial cells (HOECs) were obtained from American type culture collection (ATCC, USA) and Procell (Wuhan, China), respectively.

Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Luciferase, Western Blot, Over Expression